Qiagen, 74804, RNeasy Lipid Tissue Mini Kit (50)

50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers

Features

- Optimized lysis conditions for fatty tissues and other tissues
- High yields of total RNA without phenol contamination
- High-quality RNA for all downstream applications

Product Details

The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube Connect . Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

Performance

The RNeasy Lipid Tissue protocol integrates QIAzol lysis with RNeasy RNA isolation for high yields of total RNA from fatty tissues with an average yield of RNA from 10 mg of brain of 10 µg, and 2.4 µg of RNA from a 10 mg sample of adipose tissue. RNeasy Lipid Tissue Kit provide efficient isolation of high-quality total RNA without phenol carryover (see figure " High yields of RNA without phenol carryover "). The RNA from fatty tissues is suitable for downstream applications including real-time RT-PCR (see figure " Real-time analysis of high-quality RNA ").

Principle

RNeasy Lipid Tissue Kit is optimized for use with fatty tissues, such as brain and adipose tissue. The convenient RNeasy Lipid Tissue protocol integrates optimized phenol/guanidine-based lysis with proven RNeasy purification for isolation of high yields of high-quality total RNA. The combination of organic extraction and chaotropic disruption contributes to the high lysis efficiency of QIAzol Lysis Reagent. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.

Applications

RNeasy Lipid Tissue Kit provides easy and efficient isolation of high-quality RNA for all downstream applications, including NGS and real-time RT-PCR.