Qiagen, 959034, EZ1&2 RNA Tissue Mini Kit (48)

For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water

Features

- Purification of up to 30 µg total RNA
- Reliable processing of up to 10 mg easy-to-lyse tissues
- RNase-free setup for reliable results
- Scalable elution volumes
- Easy protocol setup

Product Details

The EZ1&2 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

The EZ1&2 RNA Tissue Mini Kit is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).

Performance

The yields and high quality of total RNA purified using the EZ1&2 RNA Tissue Mini Kit are consistent and reproducible (see figures " Efficient purification and reproducible yields " and " Consistent highly pure RNA ").

Principle

- the EZ2 Connect
- the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card

Automated purification with the EZ1&2 RNA Tissue Mini Kit requires use of one of the following:

EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1&2 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

Procedure

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1&2 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

Applications

- Gene expression analysis using real-time RT-PCR technologies
- Gene expression analysis using microarray technologies
- Oncology and pathology research

The high-quality RNA obtained using the EZ1&2 RNA Tissue Mini Kit is suited for use in many applications, such as: