Thermo Fisher, 71194400UG, HotStart-IT Binding Protein

Thermo Fisher, 71194400UG, HotStart-IT Binding Protein HotStart-IT Binding Protein is the active component in the novel hot start technology known as primer sequestration. In primer sequestration, hot start PCR methods reduce or eliminate non-specific primer-extension products formed at lower temperatures during assembly of PCR reactions. At these less stringent annealing temperatures, primers may bind non-specifically, which often leads to unwanted amplification products and primer-dimers. To resolve this problem, we have produced a high-quality DNA binding protein that is especially useful at sequestering primers at lower temperatures, making them unavailable for use by a polymerase (see figure).
Compatible Buffer: 1X PCR Reaction Buffer
For Use With (Application): Hot-start PCR
Product Type: HotStart-IT Binding Protein
Quantity: 400 μg (200 x 25 μL rxns)
Shipping Condition: Dry Ice
Unit Size: Each