Thermo Fisher, K280002, Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO™ Vector also features:
•ccdBgene for positive selection (2)
•EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection inE. coli
• SP6 promoter/primer site forin vitroRNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screeningSelected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis. Bacterial or Yeast Strain: TOP10
Cell Type: Chemically Competent
Cloning Method: Blunt TOPO™
Product Line: One Shot™
Product Type: PCR Cloning Kit
Promoter: SP6
Quantity: 25 Reactions
Vector: Blunt DNA Cloning Vectors
Format: Kit
Unit Size: Each