Thermo Fisher, K450002, TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include:
• 3´-T overhangs for direct ligation ofTaq-amplified PCR products
• Choice of T7 (pCR™2. 1-TOPO™) or T7 and SP6 (pCR™II-TOPO™) promoters forin vitroRNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
•EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection inE. coli
• Easy blue/white colony screening for selection of recombinantsTOPO™ TA Cloning™ Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis. Bacterial or Yeast Strain: TOP10
Cell Type: Chemically Competent
Cloning Method: TOPO™-TA
Product Line: One Shot™
Product Type: Cloning Kit
Promoter: T7, SP6
Quantity: 25 Reactions
Vector: TOPO-TA Cloning Vectors
Format: Kit
Unit Size: Each