Waters, 176002997, ACQUITY UPLC Protein SEC Column 450Å, 2.5 µm, 4.6 mm x 300 mm and BEH450 SEC Protein Standard Mix

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: Diol (OH)
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 5000 psi (345 Bar)
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Min: 100000
Molecular Weight Range Max: 1500000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 450 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 80
System: UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 300 mm
Carbon Load: 9 %
eCord: Yes
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein SEC Column 450Å, 2.5 µm, 4.6 mm x 300 mm and BEH450 SEC Protein Standard Mix Invest in the combination kit offered by Waters that includes both the ACQUITY UPLC Protein SEC Column as well as the BEH450 SEC Protein Standard Mix. The ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. The lab equipment is based upon the highly successful BEH particle technology and is a total system solution that will routinely deliver fast, accurate aggregate determinations up to 10 times quicker than the traditional HPLC-based SEC methods. Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 300 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. The total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker than traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume. Use the ACQUITY UPLC Protein SEC Column 450 Å, 2.5 µm, 4.6 mm x 300 mm in order to benefit from exceptional improvements in resolution, throughput, and sensitivity for assays. By monitoring the presence of protein aggregates through size-exclusion chromatography (SEC), the lab equipment increases the efficacy as the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics. This combination pack includes the BEH450 SEC Protein Standard Mix, which is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil). The Standard Mix can be used to benchmark ACQUITY UPLC BEH450 SEC, 2.5 µm Columns. This enables you to conduct regular column benchmarking and system performance checks. It is recommended that these checks be made part of the routine checkups to ensure all equipment is performing adequately. After individual testing, every column is packed with a Performance Chromatogram and Certificate of Batch Analysis so you can be certain of the quality of your lab equipment . We recommend that you also purchase the ACQUITY UPLC Protein BEH SEC Guard Column, 450 Å, 2.5 µm, 4.6 mm x 30 mm, 1/pk .

FAQ
What Is The Working pH Range For The ACQUITY UPLC Protein SEC Column? The ACQUITY UPLC Protein SEC Column listed here has an operating pH Range of 2.5-8.