Waters, 176004078, ProteinWorks Auto-eXpress Low 5 Digest Kit

"The ProteinWorks™ Auto-eXpress Low 5 Digest Kit simplifies and accelerates the digestion of immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach for protein bioanalysis. The kit includes a robust digest protocol and pre-measured, lot-traceable reagents in an automation friendly format for the denaturation, reduction, alkylation, digestion and quench of proteins in biological matrices. This product is suitable for low protein content samples (0.2 - 1.0 mg total protein) and contains the following: ProtienWorks Auto-eXpress Low Digest Ambient Kit (1) Digestion Buffer (1) Trypsin Dissolving Agent (1) Digestion Inactivation Agent (1) RapiGest SF Surfactant for low protein content samples ProteinWorks Low Digest Trypsin Kit (1) Trypsin for low protein content samples ProteinWorks Reduction Alkylation Kit (3) Reduction Agent vials. Each vial is enough for 32 samples (3) Alkylation Agent vials. Each vial is enough for 32 samples"

Specifications
Number of Analysis: 96
Type: Sample Preparation Workflow
Separation Mode: RPLC
Detection Method: MS/MS
System Type: LC-MS
UNSPSC: 41116104
Application: Protein, Peptide
Brand: ProteinWorks
Product Type: Application Kits

Additional Information
ProteinWorks Auto-eXpress Low 5 Digest Kit The Waters® ProteinWorksTM Auto-eXpress Digest Kits are adaptable, widely applicable sample preparation kits with pre-measured, lot-traceable reagents designed for precise, accurate, and reliable surrogate peptide-based LC-MS protein quantification. Proteins in immuno-purified plasma or serum can be digested using the ProteinWorks Auto-eXpress Low Digest Kit. Proteins in entire, non-immuno-purified plasma or serum can be best digested with ProteinWorks Auto-eXpress High Digest. Either 3-Step (without alkylation and reduction) or 5-Step (with alkylation and reduction) kits are available to accommodate a wide range of proteins and a number of varied surrogate peptides for quantification. The ProteinWorks™ Auto-eXpress Low 5 Digest Kit simplifies and accelerates the digestion of immuno-purified plasma & serum samples for LC/MS protein quantification via the surrogate peptide approach. The lab equipment includes a robust digest protocol and pre-measured, lot-traceable reagents in an automation-friendly format for the denaturation, reduction, alkylation, digestion, and quench of proteins in biological matrices. Explore the entire range of ProteinWorks kits offered by Waters, and shop for lab equipment using our website. This product is suitable for low protein content samples (0.2 - 1.0 mg total protein) and contains the following: Protein Works Low Digest Ambient Kit ProteinWorks Low Digest Trypsin Kit ProteinWorks Reduction Alkylation Kit You may also want to learn more in detail about the ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 30K, 1/pk . A wide usable pH range (1-12) and remarkably low pH stability are provided by the trifunctionally bound Waters ACQUITY UPLC Peptide BEH C18, wide pore (300 ) Columns. They provide extremely low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis, making them essential pieces of lab equipment for any scientist. The ACQUITY UPLC Peptide BEH Columns will enhance peptide chromatography. For best resolution, achieve narrow, symmetrical peaks and successfully separate a variety of peptides, including big and tiny, acidic and basic, and hydrophobic and hydrophilic. For the best chromatography and detection, you can also rely on formic acid and trifluoroacetic acid (TFA) to have good peak shape and retention.

FAQ
How Are Peptides Measured? The weight of the lyophilized powder, the absorbance of ultraviolet (UV) light, or amino acid analysis are the three most often used techniques for peptide quantification.