Waters, 186002032, Atlantis Silica HILIC Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

Specifications
Chemistry: HILIC
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Silica
pH Range Min: 1 pH
pH Range Max: 5 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: High
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 330
System: HPLC
Particle Technology: Silica
USP Classification: L3
Inner Diameter: 4.6 mm
Length: 150 mm
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
Atlantis Silica HILIC Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk Traditional reversed-phase chromatography makes it challenging to retain and separate highly basic polar analytes. Because of their hydrophilic nature and net positive charge at acidic pH, this occurs. The Atlantis Silica HILIC Column is based on HILIC, or Hydrophilic Interaction Chromatography, which is an alternate separation technique for separating water-soluble, basic, polar organic molecules. Peptides, metabolites, and actives are examples of these molecules. The Atlantis HILIC Silica columns are used in conjunction with high organic mobile phases (>80% acetonitrile) to retain analytes that are simply too polar for reverse-phase chromatography. In comparison to traditional chromatographic reversed-phase approaches, HILIC separation mechanisms provide orthogonal analyte selectivity. In comparison to reversed-phase HPLC columns, the Atlantis Silica HILIC Column provides better LC/ESI-MS response, direct compatibility with SPE solvents, and complementary selectivity. As metabolites are often more polar than their parent chemicals and present at substantially lower concentrations in samples, this leads to results with higher sensitivity and lower detection limits, which is extremely beneficial in drug metabolism research. In addition to this, the Atlantis Silica HILIC Column is universally compatible with all LC detectors and offers column-to-column repeatability. Peak tailing for polar bases is common when utilizing typical chromatographic reversed-phase columns; however, the Atlantis Silica HILIC Column gives improved peak morphologies for polar bases, resulting in faster and easier technique development. We recommend that you shop for lab equipment from Waters directly to ensure that you get genuine equipment. You can browse through our extensive catalog or check out our website to review the latest technological trends and available equipment for all of your lab needs. We recommend you also look into the Atlantis Silica HILIC VanGuard Cartridge, 100Å, 5 µm, 3.9 mm X 5 mm, 3/pk; Atlantis HILIC VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 3.9 mm and 4.6 mm I.D. Atlantis HILIC analytical columns containing 5 µm sorbent particles.

FAQ
What Are The Best Recommended Injection Solvents For HILIC? The Atlantis Silica HILIC Column should be used with 100 percent organic solvents. Acetonitrile, isopropanol, and methanol are examples of weak HILIC solvents. Water and dimethylsulfoxide (DMSO) should either be avoided or utilized in extremely small amounts if absolutely necessary to avoid undesirable peak shapes. Reversed-phase solid-phase extraction can be used to substitute water/DMSO with acetonitrile.