Waters, 186004505, XBridge Protein BEH C4 Column, 300 Å, 3.5 µm, 4.6 mm x 250 mm, 1/pk

The XBridge Protein BEH C4, 300 Å, 3.5 µm, 4.6 mm x 250 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications
Chemistry: C4
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 10 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Molecular Weight Range Max: 150000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 300 Å
QC Tested: Protein
Format: Column
Surface Area: 90
System: HPLC
Particle Technology: BEH
USP Classification: L26
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 8 %
UNSPSC: 41115709
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH C4 Column, 300Å, 3.5 µm, 4.6 mm X 250 mm, 10K - 500K, 1/pk Benefit from the XBridge Protein BEH C4 Column’s Protein Separation Technology which incorporates BEH technology and uses synthetic particles that offer the highest quality combined with consistent performance. The XBridge Protein BEH C4 Column is QC tested with a peptide map to ensure the stability of peptide separation methods. This is made possible using well-characterized, state-of-the-art bonding procedures for the C18 ligand. In addition to this, it guarantees predictable behavior with the variety of samples that are used in proteomics, protein characterization, and peptide synthesis. The resulting analytical column offers consistent batch-to-batch synthetic peptide and protein digest separations. The manufacturing techniques used for the lab equipment ensure stable particle structure and bonding chemistry from pH 1 to 12 and at elevated temperatures. Get exceptional separation of a wide variety of peptides using the XBridge Protein BEH C4 Column. It also delivers narrow and symmetrical peaks for maximum resolution. For optimal chromatography, the XBridge Protein BEH C4 Column provides superior peak shape and retention in formic acid and trifluoroacetic acid. To find more variants of the product listed here, products compatible with it, and to shop for lab equipment , browse through our catalog. It will also enable you to reach out to our offices worldwide and contact members of our staff should you have any queries regarding the products or the technology. You may also be interested in LCGC Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk that makes it possible for you to store your analytes, samples, and solutions in a chemically inert vial so that you get reliable and interference-free results from your analysis. All Waters LC/GC certified vials are also UV-tested by HPLC, the test used to detect trace levels of chemicals used in packaging and manufacturing processes.

FAQ
What Happens When A Protein Precipitates? Precipitation can occur due to a change in pH or if the hydrophobicity alters interactions between the protein and the aqueous environment. It can also happen through binding of salts or metals to protein functional groups such that intramolecular interactions are disrupted. As a result of this precipitation, the proteins denature, aggregate, and fall out of solution.