Waters, 186006939, ACQUITY UPLC Peptide CSH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. The VanGuard pre-column connects directly to the inlet of an eXtended Performance column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Direct-Connect
Pore Size: 130 Å
QC Tested: Peptide
Format: VanGuard Pre-column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 5 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Guards
Units per Package: 3 pk

FAQ
What Are The Best Techniques For Isolating And Cleaning Analytes? Two primary solid-phase extraction (SPE) methods are commonly employed to separate and purify target components in samples. The first method, known as retention-cleanup-elution, is utilized when the concentrations of the target chemicals are too low for reliable quantification. This approach involves retaining the analytes, cleaning up the sample matrix, and subsequently eluting the concentrated analytes for further analysis. It allows for the concentration of dilute samples and enrichment of trace chemicals. On the other hand, when the target component is present in high concentrations, a pass-through cleanup method can be chosen. In this approach, the sample is passed through the SPE sorbent to remove interfering substances, but no enrichment of the target component occurs.