Waters, 186009439, BioResolve SEC mAb Column, 200Å, 2.5 µm, 7.8 x 150 mm, 1/pk

Primarily used for legacy methods. If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution. The BioResolve SEC mAb Guard is specifically designed to extend the life of the BioResolve SEC mAb Columns. It effectively traps inadvertent injection of insoluble particulates and/or excipients sometimes present in samples and/or SEC eluents. The BioResolve SEC mAb Guard will not degrade the separation of mAb aggregates.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 4500 psi (310 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Waters
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl
Surface Area: 220
System: UHPLC, UPLC, HPLC
Particle Technology: BEH
Technique: SEC
USP Classification: L20, L33, L59
Inner Diameter: 7.8 mm
Length: 150 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115711
Application: Protein
Brand: BioResolve
Product Type: Columns
Units per Package: 1 pk

Additional Information
BioResolve SEC mAb Column, 200Å, 2.5 µm, 7.8 x 150 mm It’s well known that successful monoclonal antibody quantitation in HPLC requires great attention to detail, and the BioResolve SEC mAB Column offers that and so much more. This size-exclusion chromatography column contains 2.5-μm particles that are activated through BEH particle technology, giving you the proper platform for analysis of monoclonal antibodies. Plus, this BioResolve column is specifically designed for highly reproducible quantitation, which means you can trust it across multiple HPLC assays. To ensure the very specific and minute qualities of quantitation, the BioResolve SEC mAB Column is tested with the mAB Size Variant Standard. This is especially important in size-exclusion chromatography, as fewer ionic interactions are the ultimate goal. The results of the mAB Size Variant Standard separation and uracil efficiency of this column are included in the product box as well, so you can confidently continue to tackle the most demanding HPLC assays. Thanks to the vastly improved capabilities of protein SEC columns and LC systems, the non-denatured analysis of protein fragments is now achievable using the BioResolve SEC mAB Column. This especially comes in handy if you need to analyze the largest lgG mAB fragment because of hydrolytic degradation of the hinge region. What’s more, his column can handle monocolonal antibody monomers of high and low molecular weights, ensuring reliable and reproducible results. One of the most important considerations in mAB quantitation is system dispersion. The good news is that the BioResolve SEC mAB Column line offers a wide variety of column lengths, so it’s easy to find the right one for your application. And if you’re worried about the overall lifetime of this column, you can rest easy knowing the BioResolve SEC mAb Guard is here to help. Not only can it effectively target and trap inadvertent injections, it will also not degrade the separation of mAB aggregates. That kind of attention to HPLC column protection is loved by scientists everywhere. Column protection is only one of the many important things to consider when it comes to liquid chromatography and mAB separations. That’s why when it’s time to properly stock your laboratory with the right instruments, you should shop for lab equipment at Waters. We design all our products for scientists and by scientists. Explore our full scope of LC and MS products for sale.

FAQ
What’s the BioResolve SEC mAB Column pH Range? The BioResolve SEC mAB Column operates best between 1 and 8 pH.