Waters, 186009491, ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 μm, 2.1 x 100 mm, 1/pk

The ACQUITY Premier Peptide HSS T3, 100Å, 1.8 µm 2.1 x 100 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis

Specifications
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 1.8 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
QC Tested: Peptide
Format: Premier Column
System: UPLC, UHPLC
Particle Technology: HSS
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 11 %
eCord: Yes
UNSPSC: 41115711
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 μm, 2.1 x 100 mm, 1/pk A universal, silica-based bonded phase is employed in the sorbent of the ACQUITY Premier Peptide HSS T3 Column and is compatible with a 100% aqueous mobile phase. Your primary option for the retention and separation of polar and non-polar chemicals should be dependable lab equipment. The ACQUITY Premier Peptide HSS T3 Column is completely scalable and transferrable to HPLC separations, and it exhibits extremely little MS bleed. The trifunctional C18 alkyl phase is bonded at a ligand density that encourages polar compound retention and compatibility with aqueous mobile phases as part of the advanced T3 bonding process, which is used in the production of the ACQUITY Premier Peptide HSS T3 Column. The patented T3 end-capping technology is far more efficient than the conventional trimethyl silane (TMS) end-capping. In comparison to conventional columns, this novel end-capping with T3 bonding offers superior polar compound retention and water compatibility, as well as improved lab equipment performance, longevity, peak shape, and stability. Reversed-phase HPLC and UPLC technologies are used by the ACQUITY Premier Peptide HSS T3 Column to keep its position as the market leader. The MaxPeakTM High Performance Surfaces, a cutting-edge technology used in the ACQUITY Premier Peptide HSS T3 Column, has been developed to improve analyte recovery, sensitivity, and reproducibility by reducing analyte/surface interactions that can result in sample losses and unsatisfactory peak shape. The ACQUITY Premier Peptide HSS T3 Column is produced in a cGMP, ISO 9001-certified facility utilizing ultra-pure reagents and packaging materials created exclusively for use with the ACQUITY UPLC System. Each batch of ACQUITY packing material is further tested chromatographically for quality control using neutral, acidic, and basic analytes and the results are held to precise specification ranges to ensure great reproducible performance. A Performance Chromatogram and Certificate of Batch Analysis are delivered on the eCord Intelligent Chip along with the column for your reference after each column has undergone individual testing. You may also be interested in the mAb Tryptic Digestion Standard .

FAQ
What Is The ACQUITY Premier Peptide HSS T3 Column’s pH Range? The Waters ACQUITY Premier Peptide HSS T3 Column listed here is able to work well within the 2-8 pH range. What Is The Recommended Maximum Pressure That The ACQUITY Premier Peptide HSS T3 Column Can Tolerate? For the ACQUITY Premier Peptide HSS T3 Column, Waters recommends a maximum of 18000 psi (1240 Bar) pressure.