Waters, WAT080013, Protein-Pak SEC Column, 300Å, 10 µm, 7.5 mm X 300 mm, 10K - 500K, 1/pk

Primarily supports legacy methods. 10 µm diol-bonded gel filtration materials.

Specifications
Chemistry: SEC
Separation Mode: SEC/GPC
Particle Substrate: Polymer
pH Range Min: 2 pH
pH Range Max: 8 pH
Bonding Technology: SEC
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 500000
Particle Shape: Irregular
Particle Size: 10 µm
Endfitting Type: Parker-style
Pore Size: 300 Å
Format: Column
System: HPLC
USP Classification: L20
Inner Diameter: 7.5 mm
Length: 300 mm
UNSPSC: 41115709
Application: Protein
Brand: Protein-Pak
Product Type: Columns
Units per Package: 1 pk

Additional Information
Protein-Pak SEC Column, 300Å, 10 µm, 7.5 mm X 300 mm, 10K - 500K, 1/pk Add the Waters Protein-Pak SEC Column to your lab equipment collections as it is the column of choice for use with larger biological active molecules in the molecular weight range of Native Globular 10,000-400,000 and Random Coil 2000-150,000. The Protein-Pak SEC Column is shipped in 0.05% sodium azide in a water solution. Resolve proteins that differ in molecular weight easily by a factor of two while distinguishing proteins that differ by as little as 15% in molecular weight. The degree of resolution depends more on the sample mass and volume than the interaction between the sample and the stationary phase. As secondary interactions are most often ionic, by increasing the ionic strength of the mobile phase, they can be reduced, for instance, by using salt concentrations in the 0.2-0.5M range (NaCl). In some cases, you can also use 10-20% methanol in order to eliminate hydrophobic and other hydrogen-bonding interactions. To install the steel Protein-Pak SEC Column, start by removing the end plugs from your column with a 5/18” wrench and save them for storage when the column is removed from the system. The column outlet is indicated by an arrow on the label, which indicates the direction solvent should flow. Tighten the fittings 1/4-to-1/2 turn, but do not overtighten as this will damage the fitting seat. A properly prepared and assembled compression fitting in good conditions is all that is required. The lab equipment is based on 10 µm diol-bonded gel filtration materials and is also available in a range of pore sizes and column configurations that you can explore and shop for using our online store. You may also wish to explore similar products and those that can be used alongside this Protein-Pak SEC Column, such as the MassPrep Protein Standard Mix .

FAQ
What Sort Of Buffers Work With The Protein-Pak SEC Column? The Protein-Pak SEC Column listed here is stable in normal salt buffer solutions such as sodium sulfate, ammonium acetate, ammonium formate, phosphate buffers, tris acetate, and acetate buffers. Ionic strength equivalent to 0.1 M minimizes the ionic interaction between the protein sample and the stationary silica phase. Generally, the salt concentrations in aqueous solutions should be maintained below 0.5 M.