Abcam, ab100553, Human IL-13 ELISA Kit

Size: 1 x 96Tests
Human IL-13 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-13 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.15 pg/mL,
Range:0.16 - 40 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's IL-13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA
range.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C