Abcam, ab100593, Human MICB ELISA Kit

Size: 1 x 96Tests
Human MICB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MICB in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 75 pg/mL,
Range:0.0686 - 50 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's MICB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme linked immunosorbent assay for the quantitative measurement of Human MICB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MICB coated on a 96-well plate. Standards and samples are pipetted into the wells and MICB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MICB also known as MHC class I polypeptide-related sequence B is a protein with a molecular weight of around 42 kDa. It anchors on cell surfaces and is widely expressed in epithelial cells fibroblasts and monocytes. MICB surfaces on stressed cells but remains minimal in normal conditions indicating its involvement in cell stress response. It is an activating ligand for NKG2D receptor on natural killer (NK) cells and certain T cells playing a major role in immune surveillance.
Biological function summary
MICB contributes to immune system functioning by presenting stress-induced antigens to immune cells. It does not belong to a complex but engages in interactions critical for activating cytotoxic responses. By binding to the NKG2D receptor it helps activate NK cells and certain T cell subsets which results in the elimination of damaged or transformed cells. This immune activation is important for the surveillance against cancer and infected cells maintaining tissue homeostasis.
Pathways
MICB plays a role in the stress-induced NKG2D signaling pathway and is involved in the immune response pathway. It cooperates with proteins such as NKG2D and MICA another ligand of the NKG2D receptor to initiate immune cell-mediated cytotoxicity. These pathways activate NK cells and CD8+ T cells important for removing stressed or altered cells thereby influencing the body's defense mechanisms.
MICB has connections to cancer and autoimmune disorders. Aberrant expression of MICB appears in tumors affecting tumor immune evasion and associates with autoimmune diseases influencing immune tolerance. Dysregulation of MICB activity can affect interactions with proteins like NKG2D altering immune responses and contributing to disease progression. Therefore understanding MICB's role is critical for developing therapeutic interventions targeting immune-related diseases.