Abcam, ab100621, Human P cadherin ELISA Kit

Size: 1 x 96Tests
Human P cadherin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human P cadherin in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:2.47 - 18000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's P cadherin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human P cadherin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human P cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and P cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human P cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P cadherin also known as cadherin-3 or CDH3 is a calcium-dependent cell-cell adhesion glycoprotein. Scientists often refer to it as P-cadherin and it belongs to the cadherin protein family which includes other members like E-cadherin and N-cadherin. The molecular mass of P cadherin is approximately 120 kDa. It is mainly expressed in epithelial tissues including the basal side of scalp and mammary glands acting as an important mediator in maintaining tissue integrity.
Biological function summary
P cadherin plays an important role in facilitating cell adhesion and establishing proper tissue architecture. It often forms part of a larger complex by interacting with catenins including beta-catenin which helps link the cadherin itself to the actin cytoskeleton. Through these interactions P cadherin influences processes such as cell sorting boundary formation and cell polarization essential for embryonic development and tissue homeostasis.
Pathways
Cell adhesion and signal transduction are influenced by P cadherin through its involvement in the Wnt signaling pathway and cadherin-catenin complex. Within the Wnt pathway P cadherin interacts with other key proteins such as beta-catenin which transduces signals leading to gene expression changes. This interaction is important for controlling cellular processes including cell proliferation and differentiation.
P cadherin associates with various cancer types particularly breast and prostate cancers. Altered expression or dysfunction of P cadherin correlates with tumor progression and metastasis. Its interaction with E-cadherin may also contribute to the development and progression of skin disease hypotrichosis with juvenile macular dystrophy. Understanding the role of P cadherin within these contexts offers potential for developing targeted therapies and diagnostic tools.