Abcam, ab100696, Mouse IGF2 ELISA Kit

Size: 1 x 96Tests
Mouse IGF2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IGF2 in Mouse in Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 1.5 ng/mL,
Range:2.3 - 150 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's IGF2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF2 in cell culture supernatants.
This assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Please note that ab100696 has only been validated with cell culture supernatant samples and cannot be used with plasma samples. It is also not validated for serum samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C