Abcam, ab101500, Anti-CD8 alpha antibody [SP16]

Size: 20µL / 100µL / 500µL / 1mL
Anti-CD8 alpha antibody [SP16] (ab101500) is a rabbit monoclonal antibody detecting CD8 alpha in Flow Cytometry, IHC-P . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2010
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP16,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:C-terminus

Product details:
Product Specifications
Anti-CD8 alpha antibody [SP16] (ab101500) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cyt, IHC-P in human samples.
Anti-CD8 alpha antibody [SP16] (ab101500) specifically detects CD8 alpha (UniProt ID: P01732; Molecular weight: 24kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Quality and Validation
Anti-CD8 alpha antibody [SP16] (ab101500) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Abcam's high quality manufacturing and validation processes ensure Anti-CD8 alpha antibody [SP16] (ab101500) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD8 alpha antibody [SP16] (ab101500) has been cited over 53 times in peer reviewed journals and is trusted by the scientific community.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.