Abcam, ab101663, Recombinant human HDAC3 protein

Size: 5µg / 10µg
Recombinant human HDAC3 protein is a Human Full Length protein, in the 1 to 428 aa range, expressed in Baculovirus, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity 125 RLU/min/ng.,
Accession:O15379,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC3 or Histone Deacetylase 3 belongs to the class I histone deacetylases and plays an important mechanical role in epigenetic regulation by removing acetyl groups from histone proteins which causes chromatin condensation and gene expression modulation. Often referred to by its alternate name 3g6 HDAC3 has a molecular weight of approximately 49 kDa. The protein expresses broadly in various tissues including the brain liver and heart highlighting its widespread regulatory function across different body systems.
Biological function summary
HDAC3 acts as part of the nuclear receptor corepressor complex engaging with other subunits to modify chromatin structure and gene transcription. This protein influences various cellular processes such as cell cycle regulation and apoptosis. By altering chromatin architecture HDAC3 modulates the expression of numerous genes supporting cellular homeostasis and response to environmental signals.
Pathways
The HDAC3 protein integrates into key signaling networks including the Notch and NF-kB pathways. Within the Notch signaling pathway HDAC3 interacts with proteins like CSL modulating transcriptional responses critical for cell differentiation and developmental processes. In the NF-kB pathway it influences inflammation and immune responses by regulating the transcription of inflammatory genes showcasing its role in mediating complex signaling cascades.
HDAC3 is pivotal in the development and progression of various diseases such as cancer and neurodegenerative disorders. The dysregulation of HDAC3 activity can lead to abnormal cell proliferation and impaired cell death contributing to tumor growth. In neurodegenerative conditions HDAC3 interacts with proteins like tau potentially influencing tau-related pathologies. HDAC3 inhibitors are under investigation for therapeutic approaches to these challenging medical conditions highlighting its relevance in disease management.