Abcam, ab101666, Recombinant Human PITPN protein

Size: 100µg
Recombinant Human PITPN protein is a Human Full Length protein, in the 1 to 270 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q00169,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PITPN also known as phosphatidylinositol transfer protein alpha (PITPα) plays an important role in lipid signaling by transferring phosphatidylinositol and phosphatidylcholine between membranes. PITPN is a 35 kDa protein expressed in various tissues with significant levels in the brain and liver. It carries out its mechanical function by binding lipids within its hydrophobic pocket facilitating their transport between cellular membranes which is important for maintaining lipid equilibrium.
Biological function summary
PITPN ensures proper signal transduction and intracellular trafficking. It forms part of a complex that regulates the transfer and distribution of phospholipids. This activity is important for membrane dynamics impacting cell communication and growth. Moreover PITPN enables the formation of phosphoinositides which are essential for activating important signaling pathways related to cellular functions.
Pathways
PITPN integrates into key phosphatidylinositol signaling pathways. These pathways are critical for producing phosphoinositide second messengers that modulate a variety of cell processes. For example PITPN collaborates with proteins such as phospholipase C which operates in inositol phosphate production. This cooperation allows for careful coordination of cellular responses such as cytoskeletal rearrangement and membrane trafficking.
Abnormal PITPN function links to neural and metabolic disorders. Mutations in PITPN can result in neurological issues such as neurodegenerative diseases due to disrupted lipid homeostasis in the brain. Furthermore PITPN's interaction with proteins like insulin receptor substrates relates to insulin resistance and diabetes development. These connections highlight PITPN's potential as a biomarker or therapeutic target for managing these conditions.