Abcam, ab101827, Recombinant Human PRL-1 protein

Size: 100µg / 500µg
Recombinant Human PRL-1 protein is a Human Full Length protein, in the 1 to 170 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q93096,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRL-1 also known as phosphatase of regenerating liver-1 or DIFMU-P is an enzyme that functions as a phosphatase. It has a molecular mass of approximately 20 kDa. PRL-1 is actively expressed in various tissues including the liver and skeletal muscle where it plays significant roles in cellular processes. Mechanically it exhibits a dual specificity for both tyrosine and serine/threonine residues enabling it to dephosphorylate target proteins which can regulate diverse signaling pathways.
Biological function summary
PRL-1 plays an important role in cell proliferation differentiation and migration. Its activity impacts cellular motility making it important for physiological and pathological processes. While PRL-1 primarily acts as a monomer it can also form complexes with other proteins amplifying its biological effects. Elevation in PRL-1 expression levels correlates with enhanced migratory capacity of tumor cells suggesting a role in cancer progression.
Pathways
Various cellular functions involve PRL-1 which participates in the MAPK and PI3K/Akt pathways. These pathways are critical in regulating cell cycle and survival. PRL-1's relation to proteins like AKT1 and ERK1/2 highlights its contribution to intracellular signaling cascades that drive growth and survival responses in cells. PRL-1's phosphatase activity influences these pathways by modulating the phosphorylation status of various substrate proteins impacting downstream signaling.
Increased expression of PRL-1 relates to cancer particularly colorectal and breast cancers. Its role in promoting cell proliferation and migration makes it a target of interest for cancer research. PRL-1 is connected with proteins such as p53 and E-cadherin within these contexts correlating with the loss of cell-cell adhesion and increased invasive characteristics in tumor cells. Targeting PRL-1 could provide therapeutic benefits in managing cancer-associated pathologies.