Abcam, ab104035, Recombinant Human RAB6B protein

Size: 100µg
Recombinant Human RAB6B protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NRW1,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB6B also known as ras-related protein Rab-6B is a small GTPase with a molecular weight of approximately 24 kDa. It plays a mechanical role in intracellular trafficking processes specifically in the regulation of vesicular transport and Golgi apparatus function. RAB6B is widely expressed in human tissues with heightened levels observed in brain tissue particularly in cerebral cortex neurons and the central nervous system. This makes it important for understanding neurological processes and maintaining cellular homeostasis in such tissues.
Biological function summary
RAB6B acts in coordination with components of the vesicle transport machinery. It is not part of a large protein complex but interacts with several regulatory proteins to ensure accurate vesicle movement and docking within cells. This protein mainly influences retrograde transport from the Golgi back to the endoplasmic reticulum. This activity supports the recycling of proteins and lipids aiding in the proper maintenance of the Golgi structure and overall cellular function.
Pathways
RAB6B modulates significant pathways related to membrane trafficking. It has pronounced involvement in the retrograde vesicle transport pathway impacting the transport of cargos in neurons. RAB6B's interaction with other RAB proteins especially with members such as RAB3 and RAB8 influences synaptic vesicle exocytosis and endosomal sorting. This highlights its role in neurotransmitter release and synaptic plasticity which are important for neuronal communication.
Abnormalities in RAB6B expression and function associate with neurological disorders particularly Alzheimer's disease and Autism Spectrum Disorder. In Alzheimer's RAB6B's interaction with amyloid precursor protein (APP) influences amyloid-beta peptide generation an important event in disease pathology. In autism altered RAB6B activities potentially affect synaptic vesicle trafficking leading to impaired neural connectivity and function. Understanding these connections can provide deeper insights into the mechanisms behind these conditions.