Abcam, ab10510, Anti-RENT1/hUPF1 antibody

Size: 50µg
Goat Polyclonal RENT1/hUPF1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UPF1 aa 250-300.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human UPF1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.Q92900

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibodies were affinity purified using the peptide immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RENT1/hUPF1 also known as regulator of nonsense transcripts 1 or human UPF1 is an important factor in RNA surveillance mechanisms. It is an ATP-dependent helicase with a molecular mass of approximately 130 kDa. This protein is ubiquitously expressed in various tissues highlighting its importance across different biological systems. Mechanically UPF1 plays an essential role in the nonsense-mediated mRNA decay (NMD) pathway where it interacts with other core NMD factors to identify and degrade mRNAs containing premature stop codons.
Biological function summary
RENT1/hUPF1 ensures the quality control of mRNA by facilitating the rapid degradation of defective transcripts and thereby preventing the synthesis of potentially harmful truncated proteins. As part of the NMD complex UPF1 partners with proteins like UPF2 and UPF3 to form a surveillance mechanism that maintains cellular homeostasis. The complex cooperates to distinguish transcripts that require elimination from those necessary for normal cellular function.
Pathways
RENT1/hUPF1 integrates into essential cellular pathways beyond just NMD. The protein also links to the mRNA decay and translation initiation pathways playing significant roles alongside proteins such as SMG1 and eRF1. In these pathways UPF1 regulates gene expression by modulating mRNA stability and protein synthesis ensuring that only correctly processed mRNAs translate into proteins.
RENT1/hUPF1 connects to conditions like neurodevelopmental disorders and cancer. Dysregulation of UPF1 activity can lead to improper mRNA decay contributing to the accumulation of faulty transcripts which may drive disease pathogenesis. In particular UPF1 alterations are linked to the disruption of pathways involving key proteins like p53 which are critical in tumor suppression. Understanding UPF1's role in these disorders could present opportunities for therapeutic interventions.