Abcam, ab105459, Anti-WIPI2 antibody [2A2]

Size: 100µg
Anti-WIPI2 antibody [2A2] (ab105459) is a mouse monoclonal antibody detecting WIPI2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Over 60 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2A2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:EHPPM

Product details:
What is this antibody validated in?
Anti-WIPI2 antibody [2A2] (ab105459) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of WIPI2?
Anti-WIPI2 [2A2] (ab105459) specifically detects a band for WIPI2 (UniProt: Q9Y4P8) at a molecular weight of 49kDa.
Trusted by the scientific community
Anti-WIPI2 [2A2] (ab105459) was first used in a scientific publication in 2011 and has been cited over 60 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-WIPI2 antibody [2A2] (ab105459) has been confirmed by Western blot testing in WIPI2 Knockout HAP1 cells.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WIPI2 also known as WD repeat domain phosphoinositide-interacting protein 2 functions as a critical component in autophagy a cellular degradation process. This protein weighs approximately 46 kDa and plays an important role in the initiation of autophagosome formation by binding to phosphatidylinositol 3-phosphate on lipid bilayers. Its expression is detected in various tissues with significant abundance in skeletal muscle heart and brain. These expression patterns suggest a broad involvement in cellular regulation and maintenance.
Biological function summary
WIPI2 serves as an important scaffold in the formation of the autophagy machinery. It is part of a larger complex that includes key proteins like Atg16L1 and ULK1 which are essential in the autophagy process. WIPI2 assists in recruiting additional autophagic factors to the developing autophagosome contributing to the membrane curvature and expansion necessary for cargo sequestration. Its activity ensures the efficient turnover of damaged organelles and proteins maintaining cellular health and homeostasis.
Pathways
WIPI2 integrates into the autophagy and PI3K/Akt signaling pathways. Each of these pathways plays a significant role in cellular response to nutrient deprivation and stress. In the autophagy pathway WIPI2 directly interacts with proteins like LC3 facilitating the biogenesis of autophagosomes. Through the PI3K/Akt pathway WIPI2 supports cell survival and growth regulation linking metabolic status with autophagic responses. This interaction highlights its importance in cellular adaptation to environmental changes.
WIPI2 shows a strong connection to neurodegenerative diseases and cancer. Dysfunction in WIPI2's activity has been implicated in the progression of neurodegenerative disorders like Parkinson's Disease where impaired autophagy contributes to the accumulation of misfolded proteins. In cancer altered WIPI2 expression and function are observed linking it with tumorigenesis and cancer cell survival potentially through interactions with proteins such as mTOR and AMPK. Understanding WIPI2’s role in these conditions could lead to novel therapeutic strategies that target autophagy modulation.