Abcam, ab105636, Recombinant Human EDJ protein

Size: 10µg
Recombinant Human EDJ protein is a Human Full Length protein, in the 23 to 358 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9UBS4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Product details:
Previously labelled as DNAJB11.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDJ also known as Endoplasmic Reticulum DNAJ homolog or ERdj is a chaperone protein with a molecular mass of approximately 40 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells. EDJ plays a role in assisting unfolded or misfolded proteins to attain their correct structure. It belongs to the DNAJ family of co-chaperones and collaborates with other proteins particularly Hsp70 to ensure protein quality control within the ER.
Biological function summary
EDJ facilitates protein folding by stabilizing unfolded protein states and preventing protein aggregation. It acts as part of a larger multiprotein complex which is essential for maintaining cellular homeostasis. The chaperone activity of EDJ enables cells to cope with stress conditions such as those caused by ER stress or the accumulation of unfolded proteins. This involvement highlights its participation in the unfolded protein response (UPR) mechanism which restores normal cellular function in stressed cells.
Pathways
EDJ plays a significant role in the unfolded protein response and the heat shock response pathways. In these pathways EDJ interacts with proteins like BiP/GRP78 contributing to the regulatory mechanisms that maintain protein equilibrium within the ER. The protein helps manage these pathways by modulating the activity of stress sensors that initiate adaptive responses when proteins lose their proper folding within the ER.
EDJ has been implicated in the progression of neurodegenerative diseases and cancer. Its interaction with proteins such as ATF6 and IRE1 links it to processes that potentially lead to cell survival or apoptosis under pathological conditions. In neurodegenerative diseases mismanagement of protein folding and quality control where EDJ holds importance can result in protein aggregation. In cancer the regulation of ER stress pathways by EDJ may contribute to cancer cell survival under adverse conditions providing potential targets for therapeutic intervention.