Abcam, ab106169, Anti-SIR2 antibody

Size: 200µL
Rabbit Polyclonal SIRT2 antibody. Suitable for ICC, WB and reacts with Trypanosoma brucei samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Trypanosoma brucei brucei TREU927 SIR2rp1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Trypanosoma brucei,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Trypanosoma brucei brucei TREU927 SIR2rp1. The exact immunogen used to generate this antibody is proprietary information.Q57V41,
Specificity:ab106169 reacts specifically with the 38 kDa protein of Trypanosoma brucei

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in 200 µL of water, Purity-Whole antiserum, Purification notes-Lyophilized from 200 µl of serum., Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SIR2 protein also known as Silent mating type Information Regulation 2 homolog and sometimes called SIRT1 in humans serves as an NAD-dependent deacetylase. It weighs about 120 kDa and is expressed in various tissues including the brain heart and liver. Mechanically SIR2 removes acetyl groups from lysine residues on histones and other proteins influencing chromatin structure and gene expression. Its deacetylase activity plays a role in numerous cellular processes like DNA repair transcription and cell survival.
Biological function summary
SIR2 influences processes related to aging and metabolic regulation. It functions as a core component of the sirtuin protein family and associates with a complex that involves other sirtuins like SIRT2 and SIRT3. This protein alters the acetylation status of histones and non-histone proteins impacting cellular aging and stress resistance. By regulating mitochondrial biogenesis and oxidative stress responses SIR2 has critical roles in metabolic homeostasis and lifespan extension in model organisms.
Pathways
SIR2 participates actively in metabolic and stress-response pathways. It plays a significant role in the NAD+ salvage pathway and is implicated in the insulin/IGF-1 signaling pathway. SIR2 modulates the activity of several key proteins including the transcription factors FOXO and p53. FOXO proteins regulated by SIR2 deacetylation mediate the oxidative stress response and affect glucose metabolism. Interaction with p53 results in modulation of cell cycle arrest and apoptosis demonstrating SIR2’s influence beyond metabolism.
SIR2 has links to metabolic disorders and neurodegenerative diseases. Research shows associations between SIR2 activity and conditions like type 2 diabetes and Alzheimer’s disease. In the context of diabetes SIR2 impacts insulin sensitivity and glucose metabolism influencing the disease’s progression. In Alzheimer’s disease SIR2 interacts with APP the amyloid precursor protein affecting amyloid-beta production and neuronal survival. Understanding SIR2’s roles in these disorders could provide insights into potential therapeutic interventions.