Abcam, ab106356, Anti-REDD-1/DDIT4 antibody

Size: 100µg
Rabbit Polyclonal REDD-1/DDIT4 antibody. Suitable for ICC/IF, IHC-P and reacts with Mouse samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human DDIT4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DDIT4. The exact immunogen used to generate this antibody is proprietary information.Q9NX09

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REDD-1 also known as DDIT4 (DNA Damage Inducible Transcript 4) is a protein involved in cellular response to stress particularly hypoxic conditions. It has a molecular weight of approximately 35 kDa. This protein is expressed in various tissues including the brain liver and muscle indicating its importance in different physiological environments. REDD-1 acts as a negative regulator of the mechanistic target of rapamycin (mTOR) signaling pathway which plays an important role in cell growth and survival.
Biological function summary
The role of REDD-1 involves its participation in cellular adaptation to stress by inhibiting mTORC1 a complex within the mTOR pathway. Through this mechanism it helps cells to survive in limiting conditions by adjusting metabolic and growth signals. REDD-1 is not part of a larger protein complex but interacts closely with components of the mTOR pathway. Its regulation ensures proper cellular function under stress making it an important point for controlling cell behavior.
Pathways
REDD-1 serves as a significant regulator in the mTOR signaling and cellular stress response pathways. REDD-1 inhibits mTORC1 activity facilitating the cellular adaptation under nutrient starvation and hypoxic conditions. This inhibition affects the phosphorylation of downstream targets like S6K1 and 4EBP1 which are important for protein synthesis. Moreover REDD-1's relationship with the AMP-activated protein kinase (AMPK) adds another layer of regulation in energy balance and metabolic control.
REDD-1's dysregulation links to cancer and neurodegenerative diseases. In cancer altered REDD-1 expression affects mTORC1 signaling which can lead to unregulated cell growth and proliferation. The protein is connected to hypoxia-inducible factor (HIF) which contributes to tumor growth in hypoxic environments. In neurodegenerative diseases improper stress responses involving REDD-1 may exacerbate neuronal damage. Understanding REDD-1's interactions with mTORC1 and HIF could offer insights into potential therapeutic targets for these conditions.