Abcam, ab106936, Anti-SPAK antibody

Size: 100µg
Goat Polyclonal SPAK antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human STK39 aa 400-450.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human STK39 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.Q9UEW8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPAK also known as Ste20-related Proline-Alanine-rich Kinase is a serine/threonine protein kinase with a molecular mass of approximately 61 kDa. It plays a critical mechanical role in the regulation of ion transport and cell volume by phosphorylating a variety of ion transporters and channels. SPAK expresses in many tissues including the kidney brain and heart which highlights its multifaceted functions in different physiological contexts.
Biological function summary
SPAK integrates into cellular signaling processes by contributing to the regulation of sodium potassium and chloride transport. This kinase mainly interacts within complexes of kinase networks that include OSR1 and WNK kinases. SPAK has essential roles in mediating responses to osmotic stress and modulating ion homeostasis reflecting its participation in regulating blood pressure and neuronal excitability.
Pathways
SPAK serves a significant role in the WNK signaling pathway and the MAPK pathway. WNK kinases activate SPAK through phosphorylation allowing SPAK to modulate the activity of co-transporter proteins. This interaction is important for managing ion fluxes across membranes. Furthermore SPAK's interplay with the MAPK pathway implicates it in cellular stress responses and proliferation where it coordinates with players like p38 MAPK to influence cell survival.
Researchers have linked SPAK to conditions like hypertension and epilepsy. Its association with hypertension arises through its involvement in sodium balance where disrupted SPAK activity can lead to increased blood pressure. SPAK also connects with epilepsy via modulation of neuronal ion transport playing a part alongside other proteins such as OSR1 in maintaining neuronal excitability. Understanding SPAK's role helps in developing strategies for managing these conditions.