Abcam, ab106942, Anti-GPCR GPR81 antibody

Size: 100µg
Goat Polyclonal GPCR GPR81 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human HCAR1 aa 200-250.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HCAR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.Q9BXC0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
G protein-coupled receptor 81 (GPR81) also known as Hydroxycarboxylic acid receptor 1 (HCAR1) plays an important role in lactate signaling. It has a molecular mass of approximately 40 kDa. GPR81 is expressed mainly in adipose tissue as well as in muscle and liver cells. The receptor responds to changes in lactate levels in these tissues and influences metabolic processes accordingly.
Biological function summary
GPR81 mediates lactate sensing and regulates lipolysis. It does not form part of a larger complex but functions on its own transmitting signals inside the cell upon lactate binding. This action reduces the breakdown of lipids by inhibiting the enzyme adenylate cyclase which in turn decreases intracellular cAMP levels. By limiting lipolysis GPR81 plays a role in energy balance and metabolic homeostasis.
Pathways
GPR81 integrates into energy regulation and metabolic pathways. It is significantly involved in the lactate and lipid metabolism pathway connecting with adenylate cyclase as a downstream effector. The inhibition of adenylate cyclase reduces cAMP synthesis affecting the Protein Kinase A (PKA) signaling pathway. This pathway links GPR81 to other receptors that modulate energy homeostasis such as GPR109A.
Research connects GPR81 to obesity and type 2 diabetes. By modulating lipolysis in adipose tissue GPR81 impacts fat storage and insulin sensitivity. The receptor's activity could also indirectly influence pathways involving insulin receptor substrate (IRS) proteins key players in glucose regulation. Understanding and targeting GPR81 may provide therapeutic advantages for managing these metabolic conditions.