Abcam, ab107143, Recombinant E. coli glsA1 protein

Size: 100µg
Recombinant E. coli glsA1 protein is a Escherichia coli K-12 Full Length protein, in the 1 to 310 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P77454,
Animal free:No,
Carrier free:No,
Species:Escherichia coli K-12,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target glsA1 also known as Glutaminase 1 is an enzyme involved in the hydrolysis of glutamine to glutamate and ammonia playing an important role in nitrogen metabolism. It has a molecular mass of approximately 65 kDa. This enzyme is expressed in various tissues including the liver kidneys and brain where it assists in maintaining glutamate levels required for numerous cellular functions.
Biological function summary
GlsA1 serves an essential role in energy production and neurotransmitter cycling. As an important contributor to the glutaminase complex it ensures the conversion of glutamine into glutamate which neurons in the central nervous system use for signaling. This conversion process supports neurotransmitter balance and overall neural function impacting brain health and activity.
Pathways
The enzyme glsA1 plays a significant position in the glutaminolysis and tricarboxylic acid (TCA) cycle pathways. It assists in integrating amino acid metabolism with energy production. Through its interaction with other proteins such as GLUD1 glsA1 influences the regulation of oxidative phosphorylation. These pathways are critical for cellular energy homeostasis demonstrating the enzyme’s role in metabolic adaptability.
GlsA1 displays a link to certain cancers including glioblastoma where increased expression has been observed suggesting an association with tumor growth and survival. Moreover glsA1 is related to hepatic encephalopathy displaying connections with GLUD1 in metabolic disturbances. These associations indicate the relevance of glsA1 in understanding metabolic reprogramming in disease contexts.