Abcam, ab10805, Anti-5-methylcytosine (5-mC) antibody [33D3]

Size: 50µg
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody detecting 5-methylcytosine (5-mC) in Flow Cytometry, IP, IHC-P, IHC-Fr . - Over 170 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:33D3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Applications:Flow Cyt, IHC-P, IHC-Fr, IP, SBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Modified base 5-methylcytidine found in DNA of plants and vertebrates.Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.

Product details:
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr, IHC-P, IP, SB in human samples.
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has been cited over 177 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has high sensitivity and specificity.
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has 11 independent reviews from customers.
Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) specifically detects 5-methylcytosine (5-mC) (UniProt ID: ; Molecular weight: kDa) and is sold in 50 µg selling sizes.
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, 0.876% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-methylcytosine often referred to as 5-mC 5-methyl cytosine or 5-methylcytidine is a methylated form of the nucleobase cytosine found in the DNA of various organisms. This modification typically occurs at the carbon 5 position of the cytosine ring contributing to the epigenetic regulation of gene expression. While the mass of 5-mC itself is relatively small since it is a modified nucleotide its impact on gene regulation is significant. 5-mC is widely expressed in the genome of higher eukaryotes particularly in CpG dinucleotides where it plays a role in mammalian development and cell differentiation.
Biological function summary
Cytosine methylation affects DNA-protein interactions leading to changes in chromatin structure and the regulation of gene activity. 5-methylcytosine is an important player in the chromatin modification complex where it collaborates with other epigenetic marks to control gene expression profiles. This modified nucleotide helps to stabilize gene silencing maintaining the closed chromatin state which inhibits the transcription machinery's access to specific genomic regions. Such silencing is vital during processes like X-chromosome inactivation and imprinting.
Pathways
Researchers have identified 5-mC within two critical pathways: DNA methylation and demethylation cycle and histone modification pathway. In the DNA methylation pathway 5-mC interacts closely with proteins like DNA methyltransferases (DNMTs) which directly add methyl groups to cytosine bases. In the context of histone modification 5-mC influences the binding of methyl-CpG-binding domain (MBD) proteins which read and interpret methylated DNA marks affecting histone modification and DNA accessibility.
The dysregulation of 5-methylcytosine patterns associates prominently with cancer and neurological disorders. Abnormal methylation patterns where 5-mC is either hypermethylated or hypomethylated can lead to the silencing of tumor suppressor genes or activation of oncogenes forming a basis for cancer development. In neurological disorders altered 5-mC levels link to conditions such as Rett syndrome where the dysfunction of the associated protein MeCP2 an important reader of 5-mC contributes to the disease pathology.