Abcam, ab108251, Anti-ATG3 antibody [EPR4801]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ATG3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 28 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4801,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG3 also known as Autophagy-related protein 3 is an essential enzyme in the autophagy process. Its molecular weight is approximately 36 kDa. This protein facilitates the conjugation of LC3 (microtubule-associated protein 1A/1B-light chain 3) by acting as an E2-like enzyme. It is mainly expressed in tissues with high rates of autophagy including liver heart and brain.
Biological function summary
ATG3 plays an important role in the elongation of the autophagic membrane. It operates as a part of the autophagy machinery working closely with ATG7 an E1-like enzyme to transfer phosphatidylethanolamine (PE) to LC3 converting it into LC3-II. This lipidated form of LC3 is essential in the expansion and closure of the isolation membrane which forms the autophagosome the core structure for degradation and recycling of cellular components.
Pathways
ATG3 is a central player in the macroautophagy pathway a process critical for cellular homeostasis. It interacts with ATG7 to facilitate the lipidation of ATG8 family proteins which are important for autophagosome formation. Moreover ATG3 also links to apoptosis pathways where its balance with pro-apoptotic proteins helps regulate cell death processes.
ATG3 has been implicated in neurodegenerative diseases like Alzheimer's. This relates partly to its role in protein clearance which is essential for preventing toxic aggregations. Altered ATG3 function can lead to accumulation of misfolded proteins exacerbating disease. Moreover ATG3's interaction with proteins in the cancer-related pathways such as p53 shows its involvement in tumorigenesis through its influence on cell survival and death mechanisms.