Abcam, ab108327, Anti-APG5L/ATG5 antibody [EPR1755(2)]

Size: 20µL / 10µL-TRIAL / 100µL / 1mL
Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) is a rabbit monoclonal antibody detecting APG5L/ATG5 in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 190 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1755(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of APG5L/ATG5?
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) specifically detects a band for APG5L/ATG5 (UniProt: Q9H1Y0) at a molecular weight of 32kDa.
Trusted by the scientific community
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) was first used in a scientific publication in 2012 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-APG5L/ATG5 [EPR1755(2)] (ab108327) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327) has been confirmed by Western blot testing in ATG5 Knockout HAP1 cell line,
ab277835
Other related products
We have a range of other formats of antibody clone [EPR1755(2)] also available for your convenience: ab108327, HRP -
ab199560
, Carrier free -
ab221604
ab307842
, Carrier free -
ab307843
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.
Biological function summary
ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.
Pathways
ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.
Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.