Abcam, ab108747, Human Anti-Influenza virus A IgM ELISA Kit

Size: 1 x 96Tests
Human Anti-Influenza virus A IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Influenza virus A in human plasma and serum samples. - Colorimetric readout - 450 nm - Works on any standard plate reader - Easy results interpretation - Cut-off, positive and negative controls included
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate

Product details:
Abcam's anti-Influenza virus A IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Influenza virus A in Human serum and plasma.
A 96-well plate has been precoated with Influenza virus A antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgM sample captured in plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Influenza Virus A IgM often referred to as IgM influenza is a specific class of antibodies produced by the immune system in response to an Influenza A infection. These immunoglobulins are part of the early immune response. The molecular mass of an IgM antibody approximately reaches 970 kDa. IgM antibodies are expressed on the surface of B cells and can also be found in the bloodstream. They function by binding to the Influenza A antigens paving the way for subsequent immune responses. Tests like Influenza IgM ELISA can detect their presence serving as critical diagnostic tools.
Biological function summary
IgM antibodies play a significant role in neutralizing the virus during early infection stages. When the immune system detects the Influenza A virus it initiates the expression of these antibodies. IgM is part of the adaptive immune system and forms complexes with viral antigens to inhibit their activity and facilitate their clearance. This antibody class is often the first line of defense acting before IgG antibodies take over the sustained immune response.
Pathways
Immune cells produce IgM antibodies as part of the humoral immune response. This process involves the activation of the complement system which enhances pathogen clearance. The pathways incorporate proteins like C1q in the classic complement pathway and are interlinked with other immunoglobulins such as IgG which also participates in neutralizing pathogens after the IgM response. These pathways form an elaborate network important for effective viral elimination.
IgM antibodies against Influenza A are connected to the detection and progression of influenza infections. Elevated IgM levels typically indicate a recent or active infection and help distinguish influenza from other respiratory diseases. IgM levels decline as IgG antibodies rise marking viral elimination and recovery. In some cases the immune response including IgM might connect to broader disorders such as autoimmune conditions but direct causative relations typically involve other immune components like IgA.