Abcam, ab108892, Human PAI1 + tPA ELISA Kit

Size: 1 x 96Tests
Human PAI1 + tPA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PAI1 + tPA in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 34 pg/mL,
Range:2.4 - 8.8 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's PAI1 + tPA Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma and serum samples.
A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.
Get results in 90 minutes with Human PAI1+tPA ELISA Kit (
ab192151
) from our SimpleStep ELISA
range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols