Abcam, ab109311, Anti-EHD1 antibody [EPR4954]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal EHD1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 26 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4954,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EHD1 also known as Eps15 homology domain-containing protein 1 or EH domain-containing protein 1 is an important component in the process of endocytic recycling. EHD1 belongs to the C-terminal EHD protein family and it has a molecular mass of approximately 61 kDa. Expression of EHD1 occurs in various tissues with significant levels in the brain heart and skeletal muscle. As a member of this family EHD1 plays an authoritative role in membrane trafficking specifically in the cycling of endosomes back to the plasma membrane.
Biological function summary
In cellular transport and organization EHD1 regulates the recycling and sorting of endocytic vesicles. It functions in concert with other partners including Rab proteins and syndapin forming a complex that promotes the tubulation and fission of recycling carriers. This involvement is fundamental for maintaining cell surface receptor levels and proper cell signaling. EHD1's role is necessary to ensure cellular homeostasis and receptor-mediated processes.
Pathways
EHD1 plays an influential role in intracellular trafficking pathways particularly the endocytic recycling pathway and the trafficking of GLUT4 in adipocytes and muscle cells. In collaboration with proteins such as Rab11 and SNX5 it supports the movement of recycled membrane proteins and receptors back to the cell surface. In the GLUT4 pathway EHD1 assists in glucose uptake by facilitating the correct localization of glucose transporters on the plasma membrane which is critical for maintaining glucose homeostasis.
EHD1's dysfunction has been linked to neurodegenerative diseases and cancer. Abnormal EHD1 activity can impact neuronal signaling pathways contributing to conditions such as Alzheimer's disease. The alteration of its function can also promote disrupted recycling of growth factor receptors therefore potentially contributing to oncogenic processes. EHD1’s interactions with proteins like Rab11 and SNX5 in these contexts highlight its involvement in maintaining cellular integrity and normal function.