Abcam, ab109863, Anti-Complex IV Immunocapture antibody [31E91B82G9]

Size: 100µg
Mouse Monoclonal MTCO1 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human, Cow samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:31E91B82G9,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Cow, Human,
Applications:IP, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:100 µg ab109863 can capture at least 10 µg Complex IV (COX) from 1 mg solubilized Bovine heart mitochondria.

Product details:
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Properties and Storage Information:
Form-Liquid, Purification notes-ab109863 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex IV also known as cytochrome c oxidase is a multi-subunit enzyme complex important for the electron transport chain (ETC) in mitochondria. It consists of 13 protein subunits and has an approximate mass of 204 kDa. Complex IV is located in the inner mitochondrial membrane where it facilitates the transfer of electrons from cytochrome c to molecular oxygen reducing it to water. This process helps create a proton gradient across the inner membrane which is essential for ATP production. In high-energy demand tissues like heart and skeletal muscle expression levels of Complex IV are notably higher.
Biological function summary
Complex IV plays a critical role in cellular respiration by being the terminal enzyme of the ETC. It is part of a larger complex and its primary function is to aid in oxidative phosphorylation. This complex facilitates oxygen consumption in cells aiding ATP synthesis and influencing metabolic rate. Complex IV is involved in maintaining cellular energy homeostasis and regulation of reactive oxygen species which also contributes to cell signaling and apoptosis.
Pathways
Complex IV takes a vital role in the oxidative phosphorylation and apoptosis pathways. In oxidative phosphorylation it interacts with other ETC complexes including Complex I and II to assure efficient ATP production. The role of Complex IV in apoptosis pathways involves interactions with cytochrome c which upon release into the cytosol can trigger the apoptotic machinery. These pathways are fundamental for cellular energy production and programmed cell death ensuring proper cell function and turnover.
Complex IV dysfunction is associated with mitochondrial diseases and neurodegenerative disorders. Mitochondrial diseases such as Leigh syndrome can occur due to mutations affecting Complex IV subunits leading to impaired energy production and metabolic acidosis. In neurodegenerative disorders like Alzheimer's disease altered Complex IV activity contributes to energy deficits and increased oxidative stress. Proteins like amyloid-beta have shown connections with Complex IV alterations in Alzheimer's disease hinting at a link between mitochondrial dysfunction and the pathogenesis of such disorders.