Abcam, ab109867, Anti-ATP synthase Immunocapture antibody [12F4AD8AF8]

Size: 100µg
Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 27 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:12F4AD8AF8,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.
This antibody was tested and confirmed not suitable for WB and ICC.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification notes-The purity of ab109867 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP synthase also known as Complex V functions mechanically as a pivotal component in cellular energy production. It is a complex enzyme with a mass of approximately 600 kDa composed of multiple subunits. ATP synthase localizes primarily to the inner membrane of mitochondria though it is also found in chloroplasts and bacteria. The enzyme facilitates the synthesis of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate driven by a proton gradient established across the membrane.
Biological function summary
ATP synthase plays an essential role in cellular metabolism by converting energy stored in the proton gradient into chemical energy in the form of ATP the universal energy currency. It functions as part of the mitochondrial respiratory chain complex working alongside other complexes such as NADH dehydrogenase and cytochrome c oxidase. ATP synthase is important for energy-intensive processes as it ensures cells have adequate ATP to maintain homeostasis and perform vital functions.
Pathways
The function of ATP synthase links closely to oxidative phosphorylation and photosynthesis two important biological pathways. In oxidative phosphorylation ATP synthase acts in concert with complexes I-IV of the electron transport chain utilizing the proton gradient generated by electron transfers. The enzyme also interacts with proteins like cytochrome c and ATP/ADP translocase which facilitate electron transfer and ATP exchange respectively. These interactions highlight ATP synthase's integral role in energy production pathways.
Disruptions in ATP synthase function can lead to metabolic disorders such as mitochondrial encephalomyopathy and Leigh syndrome. These conditions involve mutations that impair ATP production and disrupt energy balance in cells. In these disorders ATP synthase remains linked to proteins implicated in mitochondrial function including cytochrome c oxidase and succinate dehydrogenase. These connections emphasize the enzyme's importance in maintaining mitochondrial integrity and energy homeostasis within cells.