Abcam, ab110217, MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric)

Size: 2 x 96Tests
MitoBiogenesis ™ In-Cell ELISA Kit (Colorimetric) is an in-cell ELISA used to identify inhibitors and activators of mitochondrial biogenesis in adherent cultured cells. - Colorimetric readout - works on any standard plate reader - Works in 96-well or 384-well plates for higher throughput - Designed to measure drug-induced effects on mitochondrial biogenesis early in the safety screening process.
Key facts
Detection method:Colorimetric,
Sample types:Adherent cells,
Reacts with:Mouse, Rat, Cow, Human,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate

Product details:
MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric) is an in-cell ELISA used to identify inhibitors and activators of mitochondrial biogenesis in adherent cultured cells.
How the assay works:
For identifying inhibitors and activators of mitochondrial biogenesis in adherent cultured cells. Each kit contains sufficient reagents to analyze two 96-well plates of fixed human, rat, mouse, or bovine cells. This kit utilizes colorimetric detection for use with standard plate readers. An alternate IR version of this kit is available which utilizes LI-COR® near-infrared IRDyes® for detection - MitoBiogenesis™ In-Cell ELISA Kit (IR) (
ab110216
In-Cell ELISA Kits use quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a 96-well plate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies and levels are quantified with enzyme-labeled secondary antibodies.
ab110217 MitoBiogenesis™ In-Cell ELISA Kit (ab110217) is designed to measure drug-induced effects on mitochondrial biogenesis early in the safety screening process. ab110217 MitoBiogenesis™ In-Cell ELISA Kit is a true duplexing 96/384-well assay that ratios both an mtDNA- and an nDNA-encoded protein in cultured or primary cells, and which requires very little sample prep and few overall steps.
Cells (human, rat or mouse) are seeded in 96- or 384-well microplates, and after exposure to experimental compounds for several cell doublings, the levels of two mitochondrial proteins are measured simultaneously in each well. The two proteins are each subunits of a different oxidative phosphorylation enzyme complex, one protein being subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the other being the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. Optionally, total protein levels can also be measured.
LI-COR®, Odyssey®, Aerius® and IRDye® are registered trademarks of LI-COR Biosciences Inc
Plates are available in our ICE (In-Cell ELISA) Support Pack (
ab111542
) which can be bought separately.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
**Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C