Abcam, ab110242, Anti-NDUFB8 antibody [20E9DH10C12]

Size: 100µg
Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) is a mouse monoclonal antibody detecting NDUFB8 in Western Blot, IHC-Fr . Suitable for Cow, Human, Mouse, Rat . - Over 200 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:20E9DH10C12,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of NDUFB8?
Anti-NDUFB8 [20E9DH10C12] (ab110242) specifically detects a band for NDUFB8 (UniProt: Q02372) at a molecular weight of 22kDa.
Trusted by the scientific community
Anti-NDUFB8 [20E9DH10C12] (ab110242) was first used in a scientific publication in 2011 and has been cited over 200 times in peer-reviewed journals.
Reviewed by scientists
Anti-NDUFB8 [20E9DH10C12] (ab110242) has over 5 independent reviews from customers.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFB8 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 is an integral component of the mitochondrial inner membrane. It is a part of the electron transport chain and plays a role in mitochondrial oxidative phosphorylation. NDUFB8 has a molecular weight of approximately 19 kDa. It is broadly expressed in tissues with high-energy demands like the heart brain skeletal muscle and liver. This expression indicates its significant involvement in energy metabolism.
Biological function summary
NDUFB8 is an accessory subunit of the mitochondrial Complex I the largest enzyme of the respiratory chain. It assists in the transfer of electrons from NADH to ubiquinone a critical step in cellular respiration that contributes to ATP production. Though not directly involved in catalysis its presence is essential for the structural integrity and assembly of Complex I. By maintaining this complex's structure NDUFB8 helps ensure efficient energy production in cells.
Pathways
NDUFB8 participates in the oxidative phosphorylation pathway playing a significant role in the mitochondrial respiratory chain. It influences the production of ATP the primary energy carrier in cells. Its association with Complex I links it to other subunits and proteins like NDUFA9 and NDUFA1 in the same pathway. These connections emphasize NDUFB8's importance within the pathway and its contribution to cellular energy management.
Dysfunction in NDUFB8 is linked to mitochondrial disorders often resulting in neurological diseases or muscle weakness such as Leigh syndrome. Mutations affecting NDUFB8 or its associated proteins like NDUFA13 could disrupt the assembly or function of Complex I leading to impaired energy production. This connection highlights the role of NDUFB8 in maintaining cellular energy balance and its impact on health when malfunctioning.