Abcam, ab110306, Anti-DLST antibody [9F4BD5]

Size: 100µg
Mouse Monoclonal DLST antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9F4BD5,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-The purity of ab110306 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DLST also known as dihydrolipoamide S-succinyltransferase is an essential component of the 2-oxoglutarate dehydrogenase complex (OGDC). It possesses a molecular mass of approximately 48 kDa. Expressed primarily in mitochondrial matrices within mammalian tissues DLST plays a mechanical role in catalyzing the transfer of succinyl groups to coenzyme-A from S-succinyldihydrolipoyllysine. The target is integral to the energy metabolism processes occurring within cells especially in tissues with high energy demands such as the heart and skeletal muscles.
Biological function summary
The DLST enzyme acts within the tricarboxylic acid (TCA) cycle also known as the Krebs cycle or citric acid cycle. It forms a critical part of the multi-enzyme complex OGDC facilitating the oxidative decarboxylation of 2-oxoglutarate an important step in the TCA cycle. Through this action DLST helps produce succinyl-CoA a molecule pivotal for energy and biosynthetic processes. The activity of this protein ensures continuous energy supply and intermediary metabolite flow within cellular respiration.
Pathways
DLST plays a significant role within both the TCA cycle and the broader metabolic pathway of oxidative phosphorylation. These are important for efficient cellular energy production connecting many metabolic reactions and facilitating ATP generation. OGDC where DLST performs its function interacts with various proteins such as E1 (2-oxoglutarate dehydrogenase) and E3 (dihydrolipoamide dehydrogenase). This interaction is necessary to efficiently process and interconvert energy substrates for cellular use.
DLST has been implicated in conditions such as neurodegenerative diseases including Alzheimer's disease. Alterations in DLST activity or expression can lead to energy production defects contributing to the metabolic disturbances observed in these conditions. Additionally mutations or dysregulation within the OGDC complex featuring DLST are associated with some forms of cancer where metabolic reprogramming occurs. In both contexts the interaction between DLST and other complex components such as E3 displays potential involvement in disease mechanisms marking it as a target worth studying for therapeutic interventions.