Abcam, ab110325, Anti-Cytochrome C antibody [37BA11]

Size: 100µg
Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody detecting Cytochrome C in Western Blot, Flow Cytometry, ICC/IF . Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat . - Over 130 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:37BA11,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human, Caenorhabditis elegans,
Applications:WB, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.
What is the molecular weight of Cytochrome C?
Anti-Cytochrome C [37BA11] (ab110325) specifically detects a band for Cytochrome C (UniProt: P99999) at a molecular weight of 12kDa.
Trusted by the scientific community
Anti-Cytochrome C [37BA11] (ab110325) was first used in a scientific publication in 2011 and has been cited over 130 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [37BA11] also available for your convenience: ab110325, Alexa Fluor® 488 -
ab154476
, HRP -
ab198489
, Alexa Fluor® 647 -
ab198583
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification technique-Affinity purification Protein G, Purification notes-ab110325 was produced in vitro using hybridomas grown in serum-free medium, and then purified by Protein G purification via FPLC and the pure IgG fraction is eluted., Storage buffer-pH: 7.4 - 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.