Abcam, ab110641, Anti-BRG1 antibody [EPNCIR111A]

Size: 100µL / 1mL
Anti-BRG1 antibody [EPNCIR111A] (ab110641) is a rabbit monoclonal antibody detecting BRG1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 190 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPNCIR111A,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, IP, ChIC/CUT&RUN-seq, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-BRG1 antibody [EPNCIR111A] (ab110641) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-BRG1 antibody [EPNCIR111A] (ab110641) was first used in a scientific publication in 2014 and has been cited over 191 times in peer reviewed journals. It's performance in Western Blot, ChIP and IHC in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-BRG1 antibody [EPNCIR111A] (ab110641) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-BRG1 antibody [EPNCIR111A] (ab110641) has been confirmed by Western Blot testing in BRG1 knockout HEK-293T cells (
ab263853
Anti-BRG1 antibody [EPNCIR111A] (ab110641) has 9 independent reviews from customers.
Anti-BRG1 antibody [EPNCIR111A] (ab110641) specifically detects BRG1 (UniProt ID: Q3TKT4; Molecular weight: 182kDa) and is sold in a convenient trial size to enable initial testing (10 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPNCIR111A -
ab215998
Antibody clone EPNCIR111A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, HRP, Alexa Fluor
647, Alexa Fluor
594, PE, APC (
ab196314
ab196315
ab196535
ab207052
ab225124
ab314953
Top cited antibody for this target with >200 citations. Validated for CUT&RUN-seq, a key application to map protein-DNA interactions on a genome-wide scale using NGS. Key target in SWI/SNF chromatin remodeling and epigenetic regulation. This antibody is crucial in cancer research, particularly in understanding BRG1's role as a tumor suppressor and its involvement in cell proliferation and gene expression. It is widely used in studies of BRG1's impact on leukaemia, breast cancer and other malignancies.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Collaborations
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BRG1 also known as SMARCA4 functions as a chromatin-remodeling enzyme. It has a molecular weight of approximately 185 kDa. BRG1 expresses in various tissues with higher expression in tissues like the brain heart and lungs. As a component of several multi-subunit complexes including the SWI/SNF complex it uses energy from ATP hydrolysis to alter chromatin structure and regulate gene expression.
Biological function summary
The BRG1 protein plays a significant role in regulating gene expression by modifying chromatin conformation. It participates in the SWI/SNF complex which is essential for transcriptional control by RNA Polymerase II. Through chromatin remodeling BRG1 influences cellular processes like cell differentiation proliferation and apoptosis. Its relevance is pronounced in dynamic cellular environments like embryonic and adult stem cells.
Pathways
BRG1 engagement in DNA damage repair and transcriptional regulation pathways underpins its biological functions. It interacts with proteins such as p53 and c-MYC which play roles in these pathways. In DNA repair pathways BRG1 supports genome integrity while in transcriptional regulation it cooperates with signaling molecules to control gene activation and repression.
Mutations or alterations in BRG1 have links to several cancers including lung and ovarian cancers. The loss of BRG1 expression often correlates with poor prognosis and tumor progression. Many tumors show an association between BRG1 and p53 mutation suggesting a complex relationship in oncogenesis. These connections highlight the importance of BRG1-specific tools like BRG1 antibodies IHC and ELISA kits for research and potential therapeutic applications.