Abcam, ab111218, Anti-DNA Polymerase theta antibody

Size: 100µg
Rabbit Polyclonal DNA Polymerase theta antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human POLQ aa 150-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human POLQ aa 150-250. The exact immunogen used to generate this antibody is proprietary information.O75417

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab111218 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DNA Polymerase theta also known as pol θ and encoded by the POLQ gene is a specialized enzyme that plays a role in DNA repair processes. It has a molecular mass of approximately 290 kDa. This enzyme functions by adding nucleotides to a DNA strand and excising incorrect bases ensuring the maintenance of genomic integrity. Pol θ is largely expressed in cells with proliferative capacity such as those found in the testes and certain types of tumor tissues. The enzyme's ability to perform template-independent nucleotide addition is unique among polymerases.
Biological function summary
DNA repair processes depend heavily on DNA Polymerase theta. This enzyme participates in a distinctive mechanism known as microhomology-mediated end-joining (MMEJ) which repairs double-strand breaks in DNA. Unlike canonical end-joining pathways MMEJ employs short homologous sequences to mediate repair where Pol θ serves a critical role. Although Pol θ acts independently it can still interact with other repair factors to ensure efficient DNA end-joining highlighting its importance.
Pathways
Maintenance of genome stability relies on pathways involving DNA Polymerase theta. The enzyme is an important component of the MMEJ pathway an alternative mechanism to the more common non-homologous end-joining (NHEJ). In this capacity Pol θ cooperates with proteins involved in DNA damage response such as BRCA1 and BRCA2 which help facilitate repair processes and support cell survival under genotoxic stress conditions.
DNA Polymerase theta has connections to cancer progression and resistance to DNA-damaging chemotherapy. Overexpression of Pol θ is common in various tumors such as breast and ovarian cancers where it promotes cell survival following DNA damage. The enzyme's interaction with the BRCA1 and BRCA2 proteins further implicates it in hereditary breast cancer syndromes suggesting a complex relationship with repair deficiencies and highlighting its potential as a therapeutic target.