Abcam, ab111729, Anti-REV3L antibody

Size: 100µg
Rabbit Polyclonal REV3L antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human REV3L aa 200-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human REV3L aa 200-300. The exact immunogen used to generate this antibody is proprietary information.O60673

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab111729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REV3L also known as DNA polymerase zeta catalytic subunit or hREV3 is an essential component of the DNA polymerase zeta (Pol ζ) complex. This protein has an approximate molecular weight of 353 kDa. It carries out its functions in several tissues with main expression in the testis thymus and ovaries although it appears in other tissues as well. Mechanically REV3L serves as the catalytic component in DNA synthesis particularly facilitating translesion DNA synthesis which allows DNA replication to bypass lesions or damage.
Biological function summary
DNA damage tolerance mechanisms involve REV3L where it plays a critical role as part of the Pol ζ complex. Pol ζ operates by extending DNA synthesis across non-instructive damages that disrupt the DNA double helix. This action involves error-prone processes but remains necessary for maintaining genomic integrity. REV3L binds with the REV7 protein in this complex to perform these translesion DNA synthesis actions shielding cells from potentially lethal damage or genomic instability due to unrepairable DNA lesions.
Pathways
The functionality of REV3L integrates into the DNA damage response and repair pathway alongside general DNA replication mechanisms. In the DNA damage response REV3L works closely with the RAD6/RAD18 pathway providing an alternate pathway for lesion bypass. The connection to other proteins like REV1 and the involvement in the broader DNA replication and repair processes highlight its important roles in cellular responses to mutagenic lesions as well as normal DNA replication processes.
Research links REV3L to cancer development particularly for tumors with high mutational rates or those exposed to genotoxic stress. The deficiency of REV3L increases susceptibility to DNA damage-induced apoptosis or uncontrolled cellular proliferation. Through its pathways REV3L also interlinks with the BRCA1 protein known for its ties to breast and ovarian cancers. The involvement of REV3L in these critical processes positions it as an important factor in understanding and potentially targeting therapy for cancers driven by DNA damage and repair pathway dysregulation.