Abcam, ab113124, Recombinant Human LAT protein

Size: 50µg
Recombinant Human LAT protein is a Human Fragment protein, in the 28 to 233 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O43561,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.
Biological function summary
LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.
Pathways
LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.
LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.