Abcam, ab113327, Human PSA ELISA Kit (KLK3)

Size: 1 x 96Tests
Human PSA ELISA Kit (KLK3) is a sandwich ELISA designed to quantify Human PSA (KLK3) with a sensitivity of 8 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 10.24 - 2500 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 8 pg/mL,
Range:10.24 - 2500 pg/mL,
Assay Platform:Microplate

Product details:
Human PSA ELISA Kit (KLK3) ab113327 is a sandwich ELISA to measure Human PSA (KLK3) in serum, plasma, cell culture supernatant with a sensitivity of 8 pg/ml.
How the assay works
This assay employs an antibody specific for Human PSA coated on a 96-well plate. Standards and samples are pipetted into the wells and PSA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PSA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PSA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human PSA ELISA Kit (KLK3) ab113327 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared biotin antibody to each well. Incubate at room temperature.
3. Add prepared Streptavidin solution. Incubate at room temperature
4. Add TMB One-Step Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Get results in 90 minutes with our SimpleStep ELISA
Human Total Prostate Specific Antigen ELISA Kit (
ab188388
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
Biological function summary
PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.
Pathways
PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.
PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.