Abcam, ab113687, Anti-PGK1 antibody [22C5D8]

Size: 250µg
Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody detecting PGK1 in Western Blot . Suitable for Saccharomyces cerevisiae . - Over 140 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:22C5D8,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Saccharomyces cerevisiae samples.
What is the molecular weight of PGK1?
Anti-PGK1 [22C5D8] (ab113687) specifically detects a band for PGK1 (UniProt: P00558) at a molecular weight of 45kDa.
Trusted by the scientific community
Anti-PGK1 [22C5D8] (ab113687) was first used in a scientific publication in 2011 and has been cited over 140 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [22C5D8] also available for your convenience: ab113687, HRP -
ab197960
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
Biological function summary
The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.
Pathways
The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.
PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.