Abcam, ab11370, Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Size: 50µL
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) is a rabbit polyclonal antibody detecting Connexin 43 / GJA1 in Western Blot, IHC-P, IHC-Fr . Suitable for Cow, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat . - Over 310 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Dog, Human, Monkey, Pig, Hamster,
Applications:IHC-P, IHC-Fr, ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GJA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P17302

Product details:
Product Specifications
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) is a rabbit polyclonal antibody and is validated for use in ICC, IHC-Fr, IHC-P, WB in cow, dog, hamster, human, monkey, mouse, pig, rat samples.
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) specifically detects Connexin 43 / GJA1 (UniProt ID: P17302; Molecular weight: 43kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) has high sensitivity and specificity.
The specificity of Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) has been confirmed by testing in knockout samples.
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) has been cited over 310 times in peer reviewed journals and is trusted by the scientific community.
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) has 31 independent reviews from customers.
Target Information
Connexin 43 (Cx43), encoded by the GJA1 gene, is a key component of gap junctions that enable direct communication between cells. It plays a vital role in maintaining tissue homeostasis and coordinating cellular functions. Abnormal Cx43 expression is linked to various diseases, including oculodentodigital dysplasia (ODDD), which affects the eyes, teeth, and fingers, and congenital heart defects. Additionally, Cx43 has been associated with cancer, where its altered expression can promote tumor progression and metastasis.
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (
ab205718
) and Goat Anti-Rabbit Alexa Fluor® 488 (
ab150077
). Or search our wide range of secondary antibodies for use with your experiment.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to connexin 43., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.
Biological function summary
This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.
Pathways
Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.
Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.