Abcam, ab114156, Recombinant Human Laminin gamma 1 protein

Size: 10µg
Recombinant Human Laminin gamma 1 protein is a Human Full Length protein, in the 1 to 39 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P11047,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Laminin gamma 1 also known as laminin-1 is an important component of the extracellular matrix. This protein has a molecular weight of about 200 kDa and is widely expressed in various tissues including epithelial and endothelial cells. Laminin gamma 1 is part of the larger laminin protein family which plays a significant role in the structural integrity of tissues. It primarily partners with other laminin chains to form a heterotrimeric complex that provides foundational support for cellular structures.
Biological function summary
Laminin gamma 1 contributes to cell adhesion differentiation and migration. It belongs to the laminin-1 complex serving as a scaffold for cells to interact with each other and with the extracellular matrix. This interaction facilitates cell signaling processes that influence cellular behavior. Additionally the laminin iHC (immunohistochemistry) technique often utilizes laminin gamma 1 to study cell-matrix interactions due to its involvement in these critical biological activities.
Pathways
Laminin gamma 1 is integral to basement membrane assembly and epithelial morphogenesis pathways. These pathways incorporate other key proteins such as integrins which mediate cell attachment to the laminin matrix. The pathway interactions involving laminin gamma 1 allow cells to respond effectively to mechanical and chemical stimuli thereby maintaining tissue homeostasis and promoting regenerative processes after injury.
Abnormalities in laminin gamma 1 expression or function associate with conditions like muscular dystrophy and certain cancers. Mutations affecting this protein can lead to compromised structural integrity and impaired cellular communication. In muscular dystrophy its relationship with proteins such as dystroglycan highlights the disruption in muscle cell adhesion and signaling. Similarly in cancer altered laminin gamma 1 expression affects tumor cell invasion and metastasis involving interactions with proteins such as matrix metalloproteinases (MMPs) which regulate extracellular matrix remodeling.